上 twice as much enzyme is used 275047

When {eq}10,000 {/eq} molecules of ATP are hydrolyzed to ADP and Pi in a test tube, about twice as much heat is liberated as when a cell hydrolyzes the same amount of ATP

Twice as much enzyme is used-Introduction Enzymes are proteins that speed up the rate of a chemical reaction without being used up Enzymes are usually specific to particular substrates The substrates in the reaction bind to active sites on the surface of the enzyme The enzymesubstrate complex then undergoes a reaction to form a product along with the original enzyme Restriction enzymes are a class of enzymes that cut DNA into fragments based upon recognizing a specific sequence of nucleotides Restriction enzymes are also known as restriction endonucleases While there are hundreds of different restriction enzymes, they all work in essentially the same way Each enzyme has what is known as a recognition

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If an enzyme is to be used to determine the concentration of substrate in a sample (eg glucose oxidase is used to measure plasma glucose), then the substrate must be the limiting factor, and the concentration of substrate must be below Km, so that the rate of formation of product increases steeply with increasing concentration of substrate, so providing a sensitive assay for theIs the slope of the initial linear portion of the curve • Rate of the reaction doubles when twice as much enzyme is used 05/31/10Prentice Hall supartono 16 c02 The MichaelisMenten Equation • Maximum velocity (Vmax ) is reached when an enzyme is

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